The protective effect of tangeretin and pomegranate separately and in combination on ethanol-induced acute gastric ulcer model

Purpose: This study was designed to find an answer the question, "Is it beneficial use pomegranate (POM) and tangeretin (TAN) separately or in combination, for prevention of acute gastric ulcer?". 
 Materials Methods: The gastroprotective effect determined by measuring levels selected inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β IL-10], lipid peroxides, enzymatic activities antioxidants tissue samples.
 Results: When all groups are written as control, ulcer, POM+EtOH, TAN+EtOH POM+TAN+EtOH, respectively; cytokine were measured 0.147, 0.24, 0.228, 0.195 0.182 pg/g protein. IL-6 levels; 16,857, 25,923, 19,797, 18,838 17,896 pg/mg TNF-α 39,916, 49.97, 44,678, 41,673 40,844 Finally, IL-10 33,496, 28,071, 29,693, 30,073 30,008 SOD 18,038, 13,731, 15,506, 14,439, 15,943. CAT 674,638, 639,964, 673,382, 664,691, 671,203. Protein carbonyl 26,799, 40,30, 33,052, 34,579 32,79. MDA found 5,239, 9,814, 6,695, 5,771 5,836. Briefly, POM TAN showed their antioxidant functions decreasing malondialdehyde (MDA), protein increasing activity superoxide dismutase (SOD) catalase (CAT). And also, these protective agents exhibited anti-inflammatory content TNF-α, IL-6, IL-1β, levels. Conclusion: Tangeretin pomegrenate have a potential against ethanol-induced ulcer that combined treatment is more than alone. In addition, this thought be due fact both flavonoids can show synergistic effect, reducing inflammation parameters